Journal
BIOSENSORS & BIOELECTRONICS
Volume 66, Issue -, Pages 155-161Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.10.073
Keywords
Gold nanocluster; Cysteamine; Thiol etching; Nanocluster-based biosensor; Thiol sensor
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Funding
- National Natural Science Foundation of China [21175010, 21275017]
- Ministry of Science and Technology of the People's Republic of China [2010DFB23160]
- Fundamental Research Funds for the Central Universities [FRF-BR-11-005A]
- SRF for ROCS, SEM
- Beijing Municipal Science and Technology Commission [z131102002813058]
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This study describes a novel Au nanocluster-based fluorescent sensor for label-free, separation-free and selective detection of cysteamine (CSH). The sensing mechanism is based on CSH etching-induced fluorescence quenching of the bovine serum albumin-protected Au-25 nanoclusters (BSAGNCs). A series of characterizations is carried out towards a better understanding of the CSH-induced fluorescence quenching of the BSAGNCs. It is found that CSH can etch the Au-25 nanoclusters, exhibiting the potent etching activity. Other thiol-containing compounds such as glutathione and cysteine and other 19 natural amino acids do not interfere with such CSH-induced etching process. The decreases in fluorescence intensity of the BSAGNCs allow sensitive detection of free CSH in the range of 500-10,000 nM. The detection limit for CSH is 150 nM (S/N=3). The spiked human serum samples can be analyzed with satisfactory results. (C) 2014 Elsevier B.V. All rights reserved.
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