Journal
BIOSENSORS & BIOELECTRONICS
Volume 63, Issue -, Pages 399-406Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.07.054
Keywords
Electrochemical biosensor; Aeromonas; Lead sulfide nanoparticles; Foodborne pathogen
Categories
Funding
- National Natural Science Foundation of China [31371768]
- Key Laboratory of Industrial Biotechnology Ministry of Education [KLIB-KF201109]
- Program for New Century Excellent Talents in Jiangnan University
- Qinglan Project
- Synergetic Innovation Center of Food Safety and quality control of Jiangsu Province
- Priority Academic Program Development of Jiangsu Higher Education Institutions
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In this paper, we reported the construction of new high sensitive electrochemical genosensor based on multiwalled carbon nanotubes-chitosan-bismuth complex (MWCNT-Chi-Bi) and lead sulfide nanopartides for the detection of pathogenic Aeromonas. Lead sulfide nanoparticles capped with 5'-(NH2) oligonucleotides thought amide bond was used as signalizing probe DNA (sz-DNA) and thiol-modified oligonucleotides sequence was used as fixing probe DNA (fDNA). The two probes hybridize with target Aeromonas DNA (tDNA) sequence (fDNA-tDNA-szDNA). The signal of hybridization is detected by differential pulse voltammetry (DPV) after electrodeposition of released lead nanoparticles (PbS) from sz-DNA on the surface of glass carbon electrode decorated with MWCNT-Chi-Bi, which improves the deposition and traducing electrical signal. The optimization of incubation time, hybridization temperature, deposition potential, deposition time and the specificity of the probes were investigated. Our results showed the highest sensibility to detect the target gene when compared with related biosensors and polymerase chain reaction (PCR). The detection limit for this biosensor was 1.0 x 10(-14) M. We could detect lower than 10(2) CFU mL(-1) of Aeromonas in spiked tap water. This method is rapid and sensitive for the detection of pathogenic bacteria and would become a potential application in biomedical diagnosis, food safety and environmental monitoring. (C) 2014 Elsevier B.V. All rights reserved.
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