Journal
BIOSENSORS & BIOELECTRONICS
Volume 74, Issue -, Pages 1038-1046Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.08.001
Keywords
DNA; Peroxidase; Thiophilicity; Gold; Platinum
Categories
Funding
- National Natural Science Foundation of China [21206107, 31200753]
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This paper reports a facile synthesis of bimetallic AuxPty nanozyme using C-rich oligonucleotide as the nucleation template, with high peroxidase-like activity and thiophilicity associated with the ratio of x:y. The efficient Au2Pt1 nanozyme possesses highly peroxidase mimicking activity with the K-m value of 0.088 mM toward 3,3',5,5'-tetramethylbenzidine and 196 mM toward hydrogen peroxide. It is the first report to explore colorimetric assays for biothiols with high sensitivity and selectivity against non-thiol-containing amino acids, on the basis of thiol-induced inhibition of peroxidase-like activities of metal nanoparticles. By using Au2Pt1 nanozyme, the limit of detection (LOD) is 3.5 nM for cysteine and 1.6 nM for homocysteine. This DNA-stabilized bimetallic AuxPty nanozyme shows a promising application in quantitative detection of biothiols in biological fluids. (C) 2015 Elsevier B.V. All rights reserved.
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