Journal
BIOSENSORS & BIOELECTRONICS
Volume 74, Issue -, Pages 207-213Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.06.043
Keywords
Molybdenum disulfide nanoribons; Gold nanoparticles; Cholesterol; Colorimetric; Peroxidase mimetic; Color wheel
Categories
Funding
- UGC
- Department of Science and Technology (DST), New Delhi, India [R/Dev/M-21-133/80164]
- CAS program in Department of Physics, B.H.U., India
- University Grants Commission (UGC), India (RJNF-JRF) [SC-252]
Ask authors/readers for more resources
In the present study, we manifest that traditionally used gold nanoparticles when supported on molybdenum disulfide nanoribbons matrix (MoS2 NRs-Au NPs) show synergistically enhanced intrinsic peroxidase like catalytic activity and can catalyze the oxidation of 3,3',5,5' tetramethyl benzidine by H2O2 to produce a highly sensitive blue shade product depending on level of free cholesterol, when tested on complex system of human serum. Further the system attests appreciable kinetics, owing to K-m value as low as 0.015 mM and better loading capacity (V-max = 6.7 x 10(-6) M s(-1)). Additionally, the proposed system is stable for weeks with ability to perform appreciably in wide pH (3-6) and temperature range (25-60 degrees C). Utilizing this potential, the present work proposes a cholesterol detection color wheel which is used along with cost effective cholesterol detection strips fabricated out of proposed MoS2 NRs-Au NPs system for quick and reliable detection of free cholesterol using unaided eye. (C) 2015 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available