Journal
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
Volume 50, Issue 5, Pages 445-452Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/10826068.2019.1706560
Keywords
Integrated method; monosodium glutamate; optimization; poly-gamma-glutamic acid; rapid screening
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In the present study, bacteria producing poly-gamma-glutamic acid were isolated from marine sands, and an efficient producer identified. gamma-PGA was rapidly screened by thin-layer chromatography and UV spectrophotometer assay. Media optimization was carried out, and for the cost-effective production of gamma-PGA, monosodium glutamate was used as the substrate for the synthesis of gamma-PGA instead of glutamic acid. Lastly, Plackett-Buman design (PB) and Response surface methodology (RSM) were used to determine significant media components and their interaction effect to achieve maximum gamma-PGA production. With this integrated method, a bacterial strain with a high yield of gamma-PGA was obtained rapidly, and the production was increased up to 37.8 g/L after optimization.
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