Physicochemical inspection and in vitro bioactivity behavior of bio-nanocomposite alginate hydrogels filled by magnesium fluoro-hydroxyapatite

The effect of magnesium fluoridated hydroxyapatite nanoparticles (Mg-FHA NPs) as ceramic species on the morphology, thermal and in vitro bioactivity properties of sodium alginate (SA) matrix was investigated. Preparation of cross-linked SA/FHA bio-nanocomposites (bio-NC)s was carried out under ultrasonic irradiations as fast and green conditions followed by cross-linking with CaCl2. The sonication influence on the dispersion of Mg-FHA in the alginate was studied thoroughly. Morphology images showed that the size of Mg-FHA NPs in the matrix reduced, considerably and the mean diameter of particles was estimated to be about 4 nm. The thermal stability of the alginate did not show significant changes by loading of 2, 6, and 10 wt% of Mg-FHA NPs. The bio-NCs exhibited the good ability of the formation of apatite in simulated body fluid. Mg-FHA growth on the surface of the samples was proved by a noteworthy increase in the phosphate absorption bands in FT-IR spectra, which shows the potential application of the obtained bio-NCs in tissue engineering.

Automated summary

The effect of magnesium fluoridated hydroxyapatite nanoparticles on the sodium alginate matrix was investigated, showing potential application in tissue engineering. The sonication influence on the dispersion of Mg-FHA in the alginate was studied thoroughly to improve the morphology and in vitro bioactivity properties of the bio-nanocomposites.