Crystal structure, DNA interaction and in vitro anticancer activity of Cu (II) and Pt(II) compounds based on benzimidazole-quinoline derivative

In this study, three transition metal compounds based on benzimidazole-quinoline ligand [Cu(pbmq)Cl-2](2) (1), [Cu(pbmq)Br-2](2) (2), and Pt(pbmq)Cl-2 (3) (where pbmq = 2-((2-(pyridin-2-yl)-1H-benzo[d]imidazol-1-yl)methyl)quinolone), were synthesized and characterized. The compounds showed much higher cytotoxicity than pbmq against the tested human cancer cell lines. Notably, compound 1 exhibited marked anticancer activity against SMMC7721 cells. Hence, compound 1 was selected for further studies to understand the underlying mechanisms of its anticancer activity. Fluorescence, CD spectroscopy studies and viscosity determination showed that compound 1 could strongly bind to calf thymus DNA (CT-DNA). The CT-DNA binding properties revealed that intercalation might be the most probable binding mode. Moreover, compound 1 exhibited DNA cleavage activity. Cell uptake implied that compound 1 could enter the cells and accumulate mainly in the nucleus. Besides, flow cytometry analysis indicated that compound 1 could induce cycle arrest and apoptosis in SMMC7721 cells. Further experiments confirmed that compound 1 triggered apoptosis in SMMC7721 cells via the intrinsic mitochondrial pathway. (C) 2020 Elsevier Ltd. All rights reserved.