Journal
BIOSENSORS & BIOELECTRONICS
Volume 65, Issue -, Pages 159-165Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.10.037
Keywords
Carbon electrodes; Alkanethiol; Diazonium salts; Biomembrane-like films; Myoglobin; p-Nitrophenylaldehyde
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Funding
- National Natural Science Foundation of China [21175126]
- Academy of Sciences for the Developing World (TWAS)
- Chinese Academy of Sciences (CAS)
- HEC Pakistan
- The Islamia University of Bahawalpur Pakistan
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Alkanethiols generally form self-assembled monolayers on gold electrodes and the electrochemical reduction of aromatic diazonium salts is a popular method for the covalent modification of carbon. Based on the reaction of alkanethiol with aldehyde groups covalently bound on carbon surface by the electrochemical reduction of aromatic diazonium salts, a new strategy for the modification of carbon electrodes with alkanethiols has been developed. The modification of carbon surface with aldehyde groups is achieved by the electrochemical reduction of aromatic diazonium salts in situ electrogenerated from a nitro precursor, p-nitrophenylaldehyde, in the presence of nitrous acid. By this way, in situ electrogenerated p-aminophenyl aldehyde from p-nitrophenylaldehyde immediately reacts with nitrous acid, effectively minimizing the side reaction of amine groups and aldehyde groups. The as-prepared alkanethiol-modified glassy carbon electrode was further used to make biomembrane-like films by casting didodecyldimethylammonium bromide on its surface. The biomembrane-like films enable the direct electrochemistry of immobilized myoglobin for the detection of hydrogen peroxide. The response is linear over the range of 1-600 mu M with a detection limit of 0.3 mu M. (C) 2014 Elsevier B.V. All rights reserved.
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