Journal
BIOSENSORS & BIOELECTRONICS
Volume 64, Issue -, Pages 241-246Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.09.007
Keywords
Cell viability; Label-free measurement; Biosensor; Refractometry; Metal clad waveguide
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Funding
- National Research Foundation of Korea (NRF) Grant - Korean Government (MSIP) [NRF-2013M3C1A8A01072922]
- KRIBB Initiative Research Program (KRIBB, Korea)
- National Research Foundation of Korea [2013M3C1A8A01072922] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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We demonstrated that a metal-clad waveguide (MCW)-based biosensor can be applied to label-free measurements of viability of adherent animal cells with osmotic stimulation in real time. After Chinese hamster ovary (CHO) and human embryonic kidney cell 293 (HEK293) cells were attached to a Concanavalin A (Con A)-modified sensor surface, the magnitudes of cell responses to non-isotonic stimulation were compared between live and dead cells. The live cells exhibited a change in the refractive index (RI) of the cytosol caused by a redistribution of water through the cell membrane, which was induced by the osmotic stimulus, but the dead cells did not. Moreover, the normalized change in the RI measured via the MCW sensor was linearly proportional to the viability of attached cells and the resolution in monitoring cell viability was about 0.079%. Therefore, the viability of attached animal cells can be measured without labels by observing the relative differences in the RI of cytosol in isotonic and non-isotonic buffers. (C) 2014 Elsevier B.V. All rights reserved.
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