Journal
BIOSENSORS & BIOELECTRONICS
Volume 64, Issue -, Pages 423-428Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.08.091
Keywords
Electrochemical aptasensor; Thrombin; Glucose oxidase; Au@Pd core-shell nanostructures; Signal amplification
Categories
Funding
- NNSF of China [21075100, 21105081, 21275119]
- Natural Science Foundation of Chongqing City [CSTC-2011BA7003, CSTC-2010BB4121]
- Ministry of Education of China [708073]
- Fundamental Research Funds for the Central Universities [XDJK2013A008, 2013A027]
- China Scholarship Council [201308500032]
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In this work, a sensitive electrochemical aptasensor for thrombin (TB) based on synergetic catalysis of enzyme and porous Au@Pd core-shell nanostructure has been constructed. With the advantages of large surface area and outstanding catalytic performance, porous Au@Pd core-shell nanostructures were firstly employed as the nanocarrier for the immobilization of electroactive toluidine blue (Tb), hemin/G-quadruplex formed by intercalating hemin into the TB aptamer (TBA) and glucose oxidase (GOx). As a certain amount of glucose was added into the detection cell, GOx rapidly catalyzed the oxidation of glucose, coupling with the local generation of H2O2 in the presence of dissolved O-2. Then, porous Au@Pd nanoparticles and hemin/G-quadruplex as the peroxidase mimics efficiently catalyzed the reduction of H2O2, amplifying the electrochemical signal and improving the sensitivity. Finally, a detection limit of 0.037 pM for TB was achieved. The excellent performance of the aptasensor indicated its promising prospect as a valuable tool in simple and cost-effective TB detection in clinical application. (C) 2014 Elsevier B.V. All rights reserved.
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