4.8 Article

Redox and catalysis 'all-in-one' infinite coordination polymer for electrochemical immunosensor of tumor markers

Journal

BIOSENSORS & BIOELECTRONICS
Volume 64, Issue -, Pages 6-12

Publisher

ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.08.024

Keywords

'All-in-one'; Redox; Catalysis; Infinite coordination polymerization; Prostate-specific antigen; Electrochemical immunosensor

Funding

  1. Natural Science Foundation of Fujian Province [2011106003]
  2. National Natural Science Foundation of China [21075019, 41176079]
  3. 973 National Basic Research Program of China [2010CB732403]
  4. Research Fund for the Doctoral Program of Higher Education of China [20103514120003]

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Prostate-specific antigen (PSA), as a glycoprotein enzyme encoded in humans by the KLK3 gene, is one of the most important biomarkers for the diagnosis and prognosis of prostate cancer. Herein, a new electrochemical immunosensor for sensitive determination of PSA was designed by using redox and catalysis 'all-in-one' infinite coordination polymer (PtNP@ICP) as signal tag on the polyamidoamine dendrimers modified electrode interface. To construct such 'all-in-one' PtNP@ICP nanostructures, the coordination polymerization was fully carried between metal ions and polydentate bridging ligands, and the PtNP was encapsulated into the ICP in the process of polymerization. The prepared PtNP@ICP nanocatalyst was characterized by transmission electron microscope (TEM), energy dispersive X-ray spectrometry (EDX), ultraviolet and visible (UV-vis) spectrophotometry and Fourier transform infrared spectroscope (FTIR). And the synthesized PtNP@ICP was utilized as signal tag for the label of PSA. With a sandwich-type immunoassay format, the conjugated signal tag on the transducer increased with the increasing PSA concentration in the sample thus enhancing the signal of the electrochemical immunosensor due to the catalytic reduction toward H2O2 of the enveloped PtNP. Under optimal conditions, the current was proportional to the logarithm of PSA concentration ranging from 0.001 to 60 ng/mL. The detection limit (LOD) was 0.3 pg/mL at 3s(B). The immunosensor displayed an acceptable reproducibility, stability and selectivity. In addition, the methodology was evaluated with human serum specimens receiving good correlation with results from commercialized enzyme-linked immunosorbent assay (ELISA) method. (C) 2014 Elsevier B.V. All rights reserved.

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