Journal
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY
Volume 28, Issue -, Pages 226-233Publisher
ELSEVIER
DOI: 10.1016/j.pdpdt.2019.09.004
Keywords
Biofilm; Streptococcus mutans; Photodynamic therapy; Hydrogen peroxide; Antimicrobial photodynamic therapy
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Funding
- National Council for Scientific and Technological Development CNPq [132211/2017-3]
- Sao Paulo Research Foundation FAPESP [2017/03263-3]
- Coordination for the Improvement of Higher Education Personnel Foundation Brazil (CAPES) Finance [001]
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Objective: This study evaluated the effect of hydrogen peroxide addition on beta-cyclodextrin-conjugated methylene blue in antimicrobial photodynamic therapy(a-PDT) in S. mutans biofilm model using laser or light emitting diode (LED) (lambda = 660 nm). Methods: A preliminary assay was performed to evaluate the cytotoxicity of hydrogen peroxide in oral fibroblasts by the colorimetric method (MTT). Afterwards, groups were divided into (n = 3, in triplicate): C (negative control), CX - chlorhexidine 0.2% (positive control), P (methylene blue/beta-cyclodextrin), H (Hydrogen Peroxide at 40 mu M), PH, L (Laser), LP, LH (Laser + Hydrogen Peroxide), LPH, LED, LEDP, LEDH, and LEDPH. The biofilm was formed in 24 h with BHI + 1% sucrose (w/v). Light irradiations were conducted with laser, 9 J, 323 J/cm(2), 113 s or with LED, 8.1 J, 8.1 J/cm(2) for 90 s. Microbial reduction was evaluated by counting the viable microorganisms of the biofilm after the respective treatments, in a selective culture medium, and laser confocal microscopy evaluation. Results: LP, LH, LPH, LEDP, LEDH, and LEDPH groups statistically reduced the counts of S.mutans compared with the C group and the log reductions were of 1.87, 1.94, 2.19, 0.91, 0.92, and 1.33, respectively; the addition of hydrogen peroxide did not potentiate the microbial reductions (LPH and LEDPH) compared with the LP and LEDP groups. Conclusion: The association of hydrogen peroxide with the conjugated beta-cyclodextrin nanoparticle as photosensitizer did not result in an enhanced effect of a-PDT; hydrogen peroxide behaved as a photosensitizer, since it reduced the number of S. mutans when associated with laser light.
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