Journal
CANADIAN JOURNAL OF MICROBIOLOGY
Volume 62, Issue 2, Pages 173-178Publisher
CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS
DOI: 10.1139/cjm-2015-0457
Keywords
Candida albicans; secreted aspartyl proteinase (SAP); virulence; resistance; pathogenicity
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Funding
- Health and Family Planning Commission of ShanXi Province, China [2014044, 201301009]
- ShanXi Province, China [2012011045-3]
- Natural Science Foundation for Young Scientists of ShanXi Province, China [2012021035-3]
- Science & Technology Innovation Foundation of ShanXi Medical University [201201013]
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The relationship between SAP2 activity and drug resistance in Candida albicans was investigated by using itraconazole-resistant and itraconazole-sensitive C. albicans isolates. The precipitation zones were measured to analyze SAP2 activity. Mice were classified into itraconazole-resistant and -sensitive C. albicans isolate groups, and a control group, with their survival and mortality rate being observed over 30 days. The relative expression levels of CDR1, CDR2, MDR1, and SAP2 were measured using RT-PCR. It was found that the secreted aspartyl proteinase activity of itraconazole-resistant C. albicans strains was significantly higher than that of itraconazole-sensitive C. albicans strains (P < 0.001). A significantly higher mortality rate was recorded for mice treated with itraconazole-resistant C. albicans than for mice treated with itraconazole-sensitive C. albicans. In regards to the CDR1, CDR2, and MDR1 genes, there was no significant difference between the 2 groups of mice. Positive correlations between SAP2 and MDR1 and between CDR1 and CDR2 were found. The high expression level of SAP2 may relate to the virulence, pathogenicity, and resistance of C. albicans.
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