A flavonone pinocembroside inhibits Penicillium italicum growth and blue mold development in 'Newhall' navel oranges by targeting membrane damage mechanism

Blue mold caused by Penicillium italicum is an important postharvest disease of citrus fruit. The antifungal activity of a flavonone pinocembroside compound obtained from the fruit of Ficus hirta Vahl., was evaluated against P. italicum. Pinocembroside showed antifungal activity against in vitro mycelial growth of P. italicum, with the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of 200 and 800 mg/L, respectively. The blue mold development on 'Newhall' navel oranges was inhibited by pinocembroside in a dose-dependent manner. Moreover, pinocembroside might exert its antifungal activity via membrane-targeted mechanism with increasing membrane permeability, reduction of antioxidant enzyme activity and acceleration of lipid peroxidation in the pathogen. This pioneering study suggested that pinocembroside suppressed postharvest blue mold by direct inhibition of P. italicum mycelial growth via membrane-targeting mechanism, thus providing a novel mode of action against traditional fungicides for controlling blue mold of citrus fruit.