Journal
BIOSENSORS & BIOELECTRONICS
Volume 74, Issue -, Pages 91-97Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.06.014
Keywords
Carbon dots; FAM-DNA; 6-mercaptopurine; Mercury ions; Fluorescence switching; Chinese yam
Categories
Funding
- Graduate Student Innovation Program [YC2014-S042]
- State Key Laboratory of Food Science and Technology of Nanchang University [SKLF-ZZA201302]
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A simple, environmentally friendly hydrothermal method was used to prepare strongly luminescent, nitrogen-doped carbon dots (NCDs) with the use of Chinese yams as a source of carbon and nitrogen. Such NCDs have an average size of 2.7 +/- 1.4 nm; they emit blue light at 420 nm and have a quantum yield of up to 9.3%. Thus, carboxyfluorescein (FAM)-DNA macro-molecules were assembled on the surfaces of the NCDs, and stabilised by strong pi-pi stacking; the so formed hybrid nano-sensors were found to have an ultra-sensitive response to 6-mercaptopurine (6-MP). A strong emission and enhancement of yellow radiation was observed from FAM. Furthermore, due to the specific interactions between DNA and Hg2+, which resulted in the formation of the T-Hg2+-T (T: thymine base) complex - a large, conjugated system, which formed between NCDs, DNA and 6-MP, was broken up. Thus, the fluorescence from FAM was quenched. The detection limits for 6-MP and Hg2+ were 0.67 and 1.26 nM, respectively. The proposed method was applied for the determination of 6-MP in human serum and Hg2+ in water samples with satisfactory results. (C) 2015 Elsevier B.V. All rights reserved.
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