Journal
BIOSENSORS & BIOELECTRONICS
Volume 69, Issue -, Pages 287-293Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.02.031
Keywords
NADH detection; Electrochemical; Gold nanoparticles; DNA tetrahedron; Graphene
Categories
Funding
- National Natural Science Foundation of China [81301293]
- National Science and Technology Major Projects for Major New Drugs Innovation and Development [2014ZX09507008]
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Dihydronicotinamide adenine dinucleotide (NADH) is a major biomarker correlated with lethal diseases such as cancers and bacterial infection. Herein, we report a graphene-DNA tetrahedron-gold nanoparticle modified gold disk electrode for highly sensitive NADH detection. By assembling the DNA tetrahedron/graphene composite film on the gold disk electrode surface which prior harnessed electrochemical deposition of gold nanoparticles to enhance the effective surface area, the oxidation potential of NADH was substantially decreased to 0.28 V (vs. Ag/AgCl) and surface fouling effects were successfully eliminated. Furthermore, the lower detection limit of NADH by the presented platform was reduced down to 1 fM, with an upper limit of 10 pM. Both the regeneration and selectivity of composite film-modified electrode are investigated and proved to be robust. The novel sensor developed here could serve as a highly sensitive probe for NADH detection, which would further benefit the field of NADH related disease diagnostics. (C) 2015 Elsevier B.V. All rights reserved.
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