Journal
NUCLEIC ACIDS RESEARCH
Volume 48, Issue 4, Pages -Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkz1204
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Funding
- Agence Nationale pour la Recherche, the Direction Generale de l'Armement [ANR-14-ASTR-0001]
- Agence Nationale pour la Recherche
- SATT Ouest-Valorisation [DV 2552]
- Direction Generale de l'Armement
- Universite de Rennes 1
- CNRS
- Agence Nationale de la Recherche (ANR) [ANR-14-ASTR-0001] Funding Source: Agence Nationale de la Recherche (ANR)
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In order to discover new antibiotics with improved activity and selectivity, we created a reliable in vitro reporter system to detect trans-translation activity, the main mechanism for recycling ribosomes stalled on problematic messenger RNA (mRNA) in bacteria. This system is based on an engineered tmRNA variant that reassembles the green fluorescent protein (GFP) when trans-translation is active. Our system is adapted for high-throughput screening of chemical compounds by fluorescence.
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