Journal
BIOSENSORS & BIOELECTRONICS
Volume 71, Issue -, Pages 30-36Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.04.007
Keywords
Multiplexed; Enzyme-free; Electrochemical; Paper electrode; Signal amplification
Categories
Funding
- National Natural Science Foundation of China [21475052, 51273084, 21175058]
- Natural Science Foundation of Shandong Province, China [ZR2012BZ002]
- Technology Development Plan of Shandong Province, China [2014GGX103012]
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Herein, an origami multiplexed enzyme-free electrochemical (EC) immunodevice is developed for the first time. Typically, ZnO nanorods (ZNRs) modified reduced graphene oxide (rGO)-paper electrode is used as a sensor platform, in which rGO improves the electronic transmission rate and ZNRs provide abundant sites for capture probes binding. Furthermore, by combining the large surface area of rGO and high catalytic activity of bovine serum protein (BSA)-stabilized silver nanoparticles (Ag@BSA) toward H2O2 reduction, rGO/Ag@BSA composites can be used as an excellent signal labels. The current signal is generated from the reduction of H2O2 and further amplified by a subsequent signal labels-promoted deposition of silver. Under optimal conditions, the proposed immunoassays exhibit excellent precision, high sensitivity and a wide linear range of 0.002-120 mIU mL(-1) for human chorionic gonadotropin, 0.001-110 ng mL(-1) for prostate-specific antigen, and 0.001-100 ng mL(-1) for carcinoembryonic antigen. The results for real sample analysis demonstrate that the newly constructed immunosensor arrays provide a simple and cost-effective method for clinical applications. (C) 2015 Elsevier B.V. All rights reserved.
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