Journal
NEUROSCIENCE LETTERS
Volume 714, Issue -, Pages -Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neulet.2019.134536
Keywords
Shh knock-in mice; Spiral ganglion neuron; Acute isolation; Membrane property; Potassium channel; Cisplatin
Categories
Funding
- National Natural Science Foundation of China [81330023, 81730028]
- Shanghai Science and Technology Commission [14DZ2260300]
Ask authors/readers for more resources
Spiral ganglion neurons (SGNs) are primary afferent auditory neurons activated by inner hair cells in mammalian cochlea. Here, for the convenience of SGN studies such as patch-clamp or single cell RNA-sequence studies, a knock-in mouse (Shh(creEGFP/+); Rosa26-Tdtomato(loxp/+)) was generated for the purpose of obtaining fluorescence SGNs. Auditory brainstem response (ABR) and Tuj1 immunohistochemistry staining were performed to verify the hearing function and the morphological characteristics. The results showed that there was no significant difference between shh and wild type mice. In electrophysiological studies, we verified a series of electrophysiological characteristics including the amplitude of sodium and potassium currents and action potential characteristics of shh and wild type mice and no significant differences were found either. From the above, shh mice have the same cell function and morphology as their littermate control wild type mice and could be used as an ideal tool to study the function and characteristics of spiral ganglion neurons. Potassium channels of SGNs play an important role in resolving time accuracy. We obtained similar amplitude of I-kappa(+) in neonatal and mature mice in the aging competition experiment, however, the density of I-kappa(+) from mature mice were significantly different from those of neonatal mice, a phenomenon that may play a key role in the nervous system. Potassium channels have been shown to contribute to apoptosis induced by cisplatin administration in various cell lines. Here we used cisplatin administration to study the ototoxicity and found that the effects of a low dose of cisplatin (0.5 mM correspond to therapeutic doses) causes a decrease in currents and is reversible after a short administration time. Moreover, we propose the activated state of potassium channels has changed but the characteristic and number remain still after cisplatin administration. The excess potassium ions may accumulate in the cell body, which had affected the firing properties and induce cytotoxicity and apoptosis. We suggest that the electrophysiological properties of acutely isolated SGNs may support further research on the mechanics of auditory propagation and ion channel pharmacology.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available