Journal
BIOSENSORS & BIOELECTRONICS
Volume 65, Issue -, Pages 341-345Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.10.055
Keywords
Quartz crystal microbalance; Rolling circle amplification; Biocatalytic precipitation; Lysozyme
Categories
Funding
- National Natural Science Foundation of China [21275003, 21275086, 21227008]
- Excellent Young Scientists Encouragement Foundation of Shandong Province [BS2011CL015]
- Scientific Technical Development Project of Qingdao [11-1-2-4-jch]
Ask authors/readers for more resources
A novel quartz crystal microbalance (QCM) assay was described for sensitive detection of protein. Lysozyme was used as a model of protein. To enhance the sensitivity of this QCM biosensor, biocatalytic precipitation (BCP) reaction combined with strand-scission cycle and rolling circle amplification (RCA) were applied together for the first time. As a result of the multi-signal amplification in this aptasensor, the detection limit for lysozyme was down to 0.3 fM. What is more, this amplified QCM biosensor also showed good selectivity and practical usage in human serum. (C) 2014 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
