Journal
BIOSENSORS & BIOELECTRONICS
Volume 73, Issue -, Pages 85-92Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.05.054
Keywords
Immunosensor; Ofloxacin; Enantioselective; Gold nanoflowers
Categories
Funding
- National Science Foundation of China [21475047, U1301214, 21105030]
- Science and Technology Planning Project of Guangdong Province [2013802080007]
- Foundation for High-level Talents in Higher Education of Guangdong Province
- Project for Construction of High-level University
- Student Innovative Project of Guangdong Province [201410564196]
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The enantioselective detection of trace amounts of ofloxacin is very important in many fields. In this work, an enantioselective and sensitive electrochemical immunosensor was constructed for the detection of chiral antibiotic ofloxacin based on a dual amplification strategy using multiwall carbon nanotubes-poly(L-lysine) as a matrix to immobilize the antigen and multi-enzyme-antibody functionalized gold nanoflowers as an electrochemical detection label. The fabrication process of the dual-amplified immunosensor was characterized by scanning electron microscopy, cyclic voltammogram and electrochemical impedance spectroscopy, respectively. After the optimization of the experimental conditions, a competitive immunoassay, i.e., the association ability with the corresponding antibody between the captured antigen and free S-OFL or R-OFL in the solution, showed that the immunosensor exhibited a sensitive response to S-OFL in the range from 0.26 to 25.6 ng/mL with a detection limit of 0.15 ng/mL as well as a sensitive response to R-OFL in the range from 0.37 to 12.8 ng/mL with a detection limit of 0.30 ng/mL. Along with the acceptable sensitivity and stability, the S-OFL or R-OFL immunosensor showed selective ability to its corresponding enantiomer, suggesting this amplification strategy may hold a potential application in the detection of OFL in food or environment. (C) 2015 Elsevier B.V. All rights reserved.
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