Journal
BIOSENSORS & BIOELECTRONICS
Volume 65, Issue -, Pages 382-389Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.10.063
Keywords
Mesoporous carbon nitride; Enzyme; Gold nanoclusters; Signal amplification; Biosensor
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Funding
- Young TopNotch Talent Support Program of China
- National Natural Science Foundation of China [51222805]
- Program for New Century Excellent Talents in University from the Ministry of Education of China [NCET-11-0129]
- Interdisciplinary Research Project of Hunan University
- China Scholarship Council (CSC) [2010843195]
- Fundamental Research Funds for the Central Universities
- Hunan University
- Hunan Provincial Innovation Foundation for Postgraduate [CX20098080]
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This work has demonstrated an amplified and selective detection platform using enzyme-scaffolded-gold nanoclusters as signal label, coupling with mesoporous carbon nitride (MCN) and gold nanoparticles (GNPs) modified glassy carbon electrode (GCE). Streptavidin-horseradish peroxidase (SA-HRP) has been integrated with gold nanoclusters (GNCs) as scaffold using a simple, fast and non-toxic method. The mechanisms of enzymatic amplification, redox cycling and signal amplification by this biosensor were discussed in detail. GNCs might perform important roles as electrocatalyst as well as electron transducer in these processes. The concentrations of reagents and the reaction times of these reagents were optimized to improve the analytical performances. Under the optimized condition, the signal response to enzyme-scaffolded-gold nanoclusters catalyzed reaction was linearly related to the natural logarithm of the target nucleic acid concentration in the range from 10(-17) M to 10(-9) M with a correlation coefficient of 0.9946, and the detection limit was 8.0 x 10(-1)8 M (S/N=3). Besides, synthesized oligonucleotide as well as Phanerochaete chrysosporium MnP fragments amplified using polymerase chain reaction and digested by restriction endonucleases were tested. Furthermore, this biosensor exhibited good precision, stability, sensitivity, and selectivity, and discriminated satisfactorily against mismatched nucleic acid samples of similar lengths. (C) Elsevier B.V. All rights reserved.
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