Journal
NATURE REVIEWS NEUROSCIENCE
Volume 21, Issue 2, Pages 61-79Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41583-019-0250-1
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Funding
- Brain/MINDS
- Basic Science and Platform Technology Program for Innovative Biological Medicine (AMED/MEXT)
- Japan Society for the Promotion of Science
- Human Frontier Science Program Research Grant Program [HFSP RGP0019/2018]
- Munich Cluster for Systems Neurology (SyNergy)
- Fritz Thyssen Stiftung
- Deutsche Forschungsgemeinschaft
- David and Lucile Packard Foundation (Packard Fellowship)
- McKnight Foundation
- US National Institutes of Health (NIH) [1-DP2-ES027992, U01MH117072]
- NCSOFT Cultural Foundation
- Koreaan Institute for Basic Science [IBS-R026-D1]
- NIH BRAIN Initiative
- NIH Office of the Director
- US National Science Foundation (NeuroNex)
- LABEX LIFESENSES [ANR-10-LABX-65, ANR-11-IDEX-0004-02]
- European Regional Development Fund of the Czech IT4Innovations National Supercomputing Center within the Czech Research, Development and Education Operational Programme [CZ.02.1.01/0.0/0.0/16_013/0001791]
- Howard Hughes Medical Institute
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State-of-the-art tissue-clearing methods provide subcellular-level optical access to intact tissues from individual organs and even to some entire mammals. When combined with light-sheet microscopy and automated approaches to image analysis, existing tissue-clearing methods can speed up and may reduce the cost of conventional histology by several orders of magnitude. In addition, tissue-clearing chemistry allows whole-organ antibody labelling, which can be applied even to thick human tissues. By combining the most powerful labelling, clearing, imaging and data-analysis tools, scientists are extracting structural and functional cellular and subcellular information on complex mammalian bodies and large human specimens at an accelerated pace. The rapid generation of terabyte-scale imaging data furthermore creates a high demand for efficient computational approaches that tackle challenges in large-scale data analysis and management. In this Review, we discuss how tissue-clearing methods could provide an unbiased, system-level view of mammalian bodies and human specimens and discuss future opportunities for the use of these methods in human neuroscience. Tissue-clearing methods are now allowing 3D imaging of intact tissues and some entire mammals. In this Review, Ueda and colleagues discuss the various tissue-clearing methods, related techniques and data analysis and management, as well as the application of these methods in neuroscience.
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