Journal
NATURE PROTOCOLS
Volume 15, Issue 3, Pages 877-924Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41596-019-0271-2
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Funding
- German Research Foundation (DFG) [BE 4492/1-2, HE 7074/1-1]
- Australian Research Council [ARC LP100200084, ARC IC160100026]
- Wesley Hospital Foundation
- AO Foundation
- Princess Alexandra Hospital Research Foundation
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This protocol describes how to establish an ovine critical-size, segmental bone defect model to study bone regeneration and reconstruction. Critical-size bone defects, which require large-volume tissue reconstruction, remain a clinical challenge. Bone engineering has the potential to provide new treatment concepts, yet clinical translation requires anatomically and physiologically relevant preclinical models. The ovine critical-size long-bone defect model has been validated in numerous studies as a preclinical tool for evaluating both conventional and novel bone-engineering concepts. With sufficient training and experience in large-animal studies, it is a technically feasible procedure with a high level of reproducibility when appropriate preoperative and postoperative management protocols are followed. The model can be established by following a procedure that includes the following stages: (i) preoperative planning and preparation, (ii) the surgical approach, (iii) postoperative management, and (iv) postmortem analysis. Using this model, full results for peer-reviewed publication can be attained within 2 years. In this protocol, we comprehensively describe how to establish proficiency using the preclinical model for the evaluation of a range of bone defect reconstruction options.
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