Journal
NATURE METHODS
Volume 17, Issue 3, Pages 287-+Publisher
NATURE RESEARCH
DOI: 10.1038/s41592-020-0762-7
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Funding
- Howard Hughes Medical Institute
- American Epilepsy Society predoctoral fellowship
- China Scholarship Council Joint PhD Training Program
- Stanford Neuroscience PhD Program training grant [5T32MH020016]
- Post-9/11 GI Bill
- Research Grants Council of the Hong Kong Special Administrative Region of China [17209017, 17259316, 17207715]
- NIH BRAIN Initiative [1U01NS103464, 1RF1MH114105, 1UF1NS107696]
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Understanding information processing in the brain requires monitoring neuronal activity at high spatiotemporal resolution. Using an ultrafast two-photon fluorescence microscope empowered by all-optical laser scanning, we imaged neuronal activity in vivo at up to 3,000 frames per second and submicrometer spatial resolution. This imaging method enabled monitoring of both supra- and subthreshold electrical activity down to 345 mu m below the brain surface in head-fixed awake mice. High-speed two-photon laser scanning microscopy using a passive laser scanner based on free-space angular-chirp-enhanced delay achieves frame rates suitable for voltage imaging in vivo in the mouse brain.
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