Journal
NATURE CHEMICAL BIOLOGY
Volume 16, Issue 4, Pages 430-+Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41589-019-0457-5
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Funding
- Ministry of Education, Culture, Sports, Science and Technology
- Japan Society for the Promotion of Science KAKENHI [JP17H06097]
- Japan Agency for Medical Research and Development
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G-protein-coupled receptors (GPCRs) are seven-transmembrane proteins mediating cellular signals in response to extracellular stimuli. Although three-dimensional structures showcase snapshots that can be sampled in the process and nuclear magnetic resonance detects conformational equilibria, the mechanism by which agonist-activated GPCRs interact with various effectors remains elusive. Here, we used paramagnetic nuclear magnetic resonance for leucine amide resonances to visualize the structure of beta(2)-adrenoreceptor in the full agonist-bound state, without thermostabilizing mutations abolishing its activity. The structure exhibited a unique orientation of the intracellular half of the transmembrane helix 6, forming a cluster of G-protein-interacting residues. Furthermore, analyses of efficacy-dependent chemical shifts of the residues near the pivotal PIF microswitch identified an equilibrium among three conformations, including one responsible for the varied signal level in each ligand-bound state. Together, these results provide a structural basis for the dynamic activation of GPCRs and shed light on GPCR-mediated signal transduction.
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