Journal
NATURE BIOTECHNOLOGY
Volume 38, Issue 2, Pages 210-+Publisher
NATURE RESEARCH
DOI: 10.1038/s41587-019-0363-0
Keywords
-
Categories
Funding
- Federal Ministry for Digital and Economic Affairs
- Federal Ministry for Transport, Innovation and Technology
- Styrian Business Promotion Agency SFG
- Standortagentur Tirol
- Government of Lower Austria
- ZIT - Technology Agency of the City of Vienna through the COMET Funding Program
- Austrian Science Fund [FWF W1224]
Ask authors/readers for more resources
The methylotrophic yeast Pichia pastoris is widely used in the manufacture of industrial enzymes and pharmaceuticals. Like most biotechnological production hosts, P. pastoris is heterotrophic and grows on organic feedstocks that have competing uses in the production of food and animal feed. In a step toward more sustainable industrial processes, we describe the conversion of P. pastoris into an autotroph that grows on CO2. By addition of eight heterologous genes and deletion of three native genes, we engineer the peroxisomal methanol-assimilation pathway of P. pastoris into a CO2-fixation pathway resembling the Calvin-Benson-Bassham cycle, the predominant natural CO2-fixation pathway. The resulting strain can grow continuously with CO2 as a sole carbon source at a mu(max) of 0.008 h(-1). The specific growth rate was further improved to 0.018 h(-1) by adaptive laboratory evolution. This engineered P. pastoris strain may promote sustainability by sequestering the greenhouse gas CO2, and by avoiding consumption of an organic feedstock with alternative uses in food production. A yeast species used to produce proteins and chemicals is engineered to grow solely on the greenhouse gas CO2.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available