Evaluating four modes of extraction to analyze bioactive compounds in Garcinia brasiliensis (bacupari) by high-performance liquid chromatography diode-array detection (HPLC-DAD)

The prior study of the extraction path is fundamental to optimize the required time and to define the most suitable solvent to extract and determine an analyte of interest in a complex sample. This study aimed to evaluate four extraction modes; solvent sequence in Soxhlet equipment (SES), and by maceration (SEM), direct extraction with ethanol by maceration (EEM), and in Soxhlet equipment (EES), and determine Garcinia brasiliensis bioactive compounds using a validated high-performance liquid chromatography diode-array detection (HPLC-DAD) method. Fukugetin, fukugetina-7''-O-beta-D-glucoside, norathyriol, guttiferone A, and 7-epiclusianone were identified and quantified with authentic standards. Among all four modes applied to extract the main bioactive. From HPLC profile, it was observed that the highest levels of 7-epiclusianone (344.1 mg/g) and guttiferone A (142.8 mg/g) were found in the N-hexane fraction using SEM mode, whereas the highest levels of fukugetin (44.95 mg/g) and norathyriol (3.95 mg/g dry weight) in the ethyl acetate fraction using SES mode.

Automated summary

This study evaluated four extraction modes to determine bioactive compounds of Garcinia brasiliensis, with different modes yielding different highest levels of bioactive components.