A new triterpenoid and other constituents with cytotoxic activity from the roots of Sanguisorba officinalis L

Sanguisorba officinalis L. is a traditional herbal plant that belongs to the genus Sanguisorba and the family Rosaceae. A new ursane-type triterpenoid, 3-oxo-urs-11, 13(18)-dien-19, 28-olide (1), two known ursane-type triterpenoids (3 - 4) and three known oleanane-type triterpenoids (2, 5 - 6) were isolated from the roots of S. officinalis by silica gel column and MPLC. Their structures were identified by interpretation of spectroscopic data (1 D NMR, 2 D NMR, HR-ESI-MS) and comparison with those reported in the literature. Compound 2 was isolated from the Rosaceae family, compounds 3-5 were obtained from the genus Sanguisorba, and compound 6 was obtained from the S. officinalis for the first time. Additionally, all of the isolated compounds were evaluated for their cytotoxic activity against three human cancer cells. Compound 3 showed better cytotoxic activity against A549, HeLa, SK-Hep1 cells than the other compounds with IC50 values of 48.58 +/- 1.88, 47.84 +/- 2.01, 42.31 +/- 2.43 mu M, respectively.

Automated summary

A new ursane-type triterpenoid and known triterpenoids were isolated from the roots of Sanguisorba officinalis, with compound 3 showing the best cytotoxic activity against human cancer cells among all compounds evaluated.