Journal
NANOTECHNOLOGY
Volume 31, Issue 19, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/1361-6528/ab6fdf
Keywords
DNA; nanomachine; metal ions; fluorescence; biosensors
Funding
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- National Natural Science Foundation of China [31701683]
- Young and Middle-Aged Teachers Education Scientific Research Project of Fujian province [JAT170079]
- China Scholarship Council (CSC) scholarship [201806655028]
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We herein report a DNA hairpin structure containing a polycytosine loop region, and this hairpin can operate like a nanomachine allowing independently controlled stability of the i-motif loop and duplex stem region. This was made possible by the opposite salt-dependent stability of DNA duplex and hairpin, thus providing a new method for designing molecular devices or switches design. A singly-labeled fluorescent method was used to measure the stability of an i-motif DNA in the presence of various metal ions. Salt in general destabilizes the i-motif but stabilizes duplex DNA, allowing us to engineer an i-motif containing hairpin for modulating the stability of each secondary structure independently.
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