Journal
NANO RESEARCH
Volume 13, Issue 5, Pages 1427-1433Publisher
TSINGHUA UNIV PRESS
DOI: 10.1007/s12274-020-2678-z
Keywords
nanoenzyme; graphene quantum dot; heme active site; peroxidase mimetic; enzymatic microenvironment
Categories
Funding
- National Key R&D Program nanotechnology special focus [2016YFA0201600]
- National Natural Science Foundation of China [21422303, 21573049, 21872043, 81602643]
- Beijing Natural Science Foundation [2142036]
- Knowledge Innovation Program, Youth Innovation Promotion Association
- Special Program of One Belt One Road of CAS
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The development of high-efficiency peroxidase mimetics is highly desirable in view of high cost and low stability of natural enzymes. From the perspective of mimicking active site microenvironment at low cost, we herein report a novel histidine-functionalized graphene quantum dot (His-GQD)/hemin complex, which exhibits the highest catalytic rate for the peroxidase-based chromogenic reaction among the hemin-containing mimetics reported so far. Also, our peroxidase mimetic shows excellent tolerance to strongly acidic conditions and can function in a wide temperature range. Lineweaver-Burk plots and comprehensive electron paramagnetic resonance analysis reveal a ping-pong type catalytic mechanism for this mimetic. In addition, His-GQD/hemin demonstrates high efficiency and accuracy in detecting H2O2 and blood glucose. Our work provides an effective design of artificial enzymes for practical applications.
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