Journal
MOLECULES
Volume 25, Issue 3, Pages -Publisher
MDPI
DOI: 10.3390/molecules25030651
Keywords
lipase from Pseudomonas fluorescens; single-walled carbon nanotubes; biodiesel production
Funding
- National Authority for Scientific Research and Innovation (ANCSI)
- European Regional Development Fund, Competitiveness Operational Program 2014-2020 (POC, Priority Axis 1, Action 1.1), grant NEMSyB [P37_273, MySMIS 103413]
- project Entrepreneurship for Innovation through Doctoral and Postdoctoral Research [POCU/360/6/13/123886]
- European Social Fund, through the Operational Program for Human Capital 2014-2020
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The Amano lipase from Pseudomonas fluorescens (L-AK) was covalently immobilized on various carbon nanomaterials (functionalized single-walled carbon nanotubes and graphene oxide) and tested for biodiesel production. Using the most active lipase preparation (covalently immobilized L-AK on SwCNT(NH2) derivatized with glycerol diglycidyl ether) under optimal conditions, quasi-complete conversion (>99%) of sunflower oil was obtained after only 4 h reaction time. Moreover, the biocatalyst maintained more than 99% of its initial activity in the batch system after multiple recycling experiments.
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