Journal
MOLECULAR MICROBIOLOGY
Volume 113, Issue 2, Pages 430-451Publisher
WILEY
DOI: 10.1111/mmi.14429
Keywords
mRNA; Trypanosoma; translation; binding; RNASeq
Categories
Funding
- Deutsche Forschungsgemeinschaft [Cl112/28-1]
- Alexander von Humboldt-Stiftung
Ask authors/readers for more resources
ZC3H20 and ZC3H21 are related trypanosome proteins with two C(x)(8)C(x)(5)C(x)(3)H zinc finger motifs. ZC3H20 is present at a low level in replicating mammalian-infective bloodstream forms, but becomes more abundant when they undergo growth arrest at high density; ZC3H21 appears only in the procyclic form of the parasite, which infects Tsetse flies. Each protein binds to several hundred mRNAs, with overlapping but not identical specificities. Both increase expression of bound mRNAs, probably through recruitment of the MKT1-PBP1 complex. At least 28 of the bound mRNAs decrease after depletion of ZC3H20, or of ZC3H20 and ZC3H21 together; their products include procyclic-specific proteins of the plasma membrane and energy metabolism. Simultaneous depletion of ZC3H20 and ZC3H21 causes procyclic forms to shrink and stop growing; in addition to decreases in target mRNAs, there are other changes suggestive of loss of developmental regulation. The bloodstream-form-specific protein RBP10 controls ZC3H20 and ZC3H21 expression. Interestingly, some ZC3H20/21 target mRNAs also bind to and are repressed by RBP10, allowing for dynamic regulation as RBP10 decreases and ZC3H20 and ZC3H21 increase during differentiation.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available