Journal
MICROCHIMICA ACTA
Volume 187, Issue 1, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-019-4036-y
Keywords
Boronate affinity adsorbent; Magnetic assisted miniaturized dispersive solid-phase extraction; Ortho-phenols; Receptor agonists; Neurotransmitters; Adenosine; Urine sample; HPLC with fluorometric detection
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Funding
- National Natural Science Foundation of China [21775121, 21974106]
- Specialized Research Fund for the Doctoral Program of Higher Education [20126101120023]
- Discipline Innovation Team Program of Shaanxi University of Chinese Medicine [2019-YL10]
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Amino-functionalized polyhedral oligomeric silsesquioxanes (POSS-8NH(2)) were covalently bound to the surface of polydopamine-coated magnetized graphene oxide. It was then reacted with 4-formylphenylboronic acid to prepare a cubic boronic acid-bonded magnetic graphene oxide adsorbent. The new adsorbent exhibits better selectivity and much higher adsorption capacity for ortho-phenols over adsorbents where small boronic ligands are directly bound to the surface of the material. It is shown to enable selective and faster enrichment of the catecholamines epinephrine (EP), dopamine (DA) and isoprenaline (IP) with high selectivity over many potential interferents that can occur in urine. The analytes were then quantified by HPLC with fluorometric detection. Under optimal conditions, response is linear (R-2 >= 0.9907), limits of detection are low (0.54-2.3 ng center dot mL(-1)), and reproducibility is acceptable (inter- and intra-day assay RSDs of <= 10.9%). The method was successfully applied to the determination of endogenous EP and DA and exogenous IP in urine samples.
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