Journal
MICROCHIMICA ACTA
Volume 187, Issue 1, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-019-4003-7
Keywords
Aptamer-AuNPs probes; Catalytic growth; Alignment device; Fiber spectrometer; Plant hormone; Varied morphologies; Absorption spectra; Peak wavelength shift
Categories
Funding
- National Natural Science Foundation of China [31671581]
- Natural Science Foundation of Henan Province [162300410143]
- Project of the Education Department of Henan Province [16A150011, 14A210010]
- China Postdoctoral Science Foundation [2017 M612399]
- Project of Science and Technology of Henan Province [172102310244, 182102110427, 182102110319]
- Science and Technology Innovation Project of Henan Agricultural University [KJCX2016A08, KJCX2018A09]
- Henan International Joint Laboratory of Laser Technology in Agriculture Sciences
Ask authors/readers for more resources
A multi-channel localized surface plasmon resonance system is described for absorptiometric determination of abscisic acid (ABA). The system is making use of gold nanoparticles and consists of a broadband light source, amulti-channel alignment device, and a fiber spectrometer. The method is based on the specific interaction between an ABA-binding aptamer and ABA. This induces the growth of gold nanoparticles (AuNPs) functionalized with a polyadenine-tailed aptamer that act as optical probes. Different concentrations of ABA give rise to varied morphologies of grown AuNPs. This causes a change of absorption spectra which is recorded by the system. ABA can be quantified by measurement of the peak wavelength shifts of grown AuNPs. Under optimized conditions, this method shows a linear relationship in the 1 nM to 10 mu MABA concentration range. The detection limit is 0.51 nM. The sensitivity of the ABA assay is strongly improved compared to the method based on salt-induced AuNP aggregation. This is attributed to the use of a poly-A-tailed aptamer and the catalytic ability of AuNPs. In the actual application, the ABA concentration of ABA in fresh leaves of rice is measured with the maximum relative error of 8.03% in comparison with the ELISA method.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available