SubsurfaceStappia: Success Through Defence, Specialisation and Putative Pressure-Dependent Carbon Fixation

Diverse microbial communities living in subsurface coal seams are responsible for important geochemical processes including the movement of carbon between the geosphere, biosphere and atmosphere. Microbial conversion of the organic matter in coal to methane involves a complex assemblage of bacteria and archaea working in syntrophic relationships. Despite the importance and value of this microbial process, very few of the microbial taxa have defined metabolic or ecological roles in these environments. Additionally, the genomic features mediating life in this chemically reduced, energy poor, deep subsurface environment are not well characterised. Here we describe the isolation and genomic and catabolic characterisation of three alphaproteobacterialStappia indicaspecies from three coal basins across Australia.S. indicagenomes from coal seams were compared with those from closely relatedS. indicaisolated from diverse surface waters, revealing a coal seam-specific suite of genes associated with life in the subsurface. These genes are linked to processes including viral defence, secondary metabolite production, polyamine metabolism, polypeptide uptake membrane transporters and putative energy neutral pressure-dependent CO(2)fixation. This indicates that subsurfaceStappiahave diverse metabolisms for biomass recycling and pressure-dependent CO(2)fixation and require a suite of defensive and competitive strategies relative to their surface-dwelling relatives.