Journal
METABOLIC ENGINEERING
Volume 56, Issue -, Pages 39-49Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymben.2019.08.011
Keywords
Corynebacterium glutamicum; Poly-gamma-glutamic acid; Glutamic acid; PGA synthase; Glutamate racemase
Categories
Funding
- Center for Biotechnology and Interdisciplinary Studies at RPI
- Program of Introducing Talents of Discipline to Universities [111-2-06]
- National Key Research and Development Program of China [2018YFA0900303]
- International Joint Research Laboratory for Engineering Synthetic Biosystems for Intelligent Biomanufacturing at Jiangnan University
- Six Talent Peaks Project in Province [2015-SWYY-006]
- Top-notch Academic Programs Project of Jiangsu Higher Education Institutions (TAPP)
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gamma-Polyglutamic acid (gamma-PGA) is a biodegradable polymer naturally produced by Bacillus spp. that has wide applications. Fermentation of gamma-PGA using Bacillus species often requires the supplementation of L-glutamic acid, which greatly increases the overall cost. Here, we report a metabolically engineered Corynebacterium glutamicum capable of producing gamma-PGA from glucose. The genes encoding gamma-PGA synthase complex from B. subtilis (pgsB, C, and A) or B. licheniformis (capB, C, and A) were expressed under inducible promoter P-tac in a L-glutamic acid producer C. glutamicum ATCC 13032, which led to low levels of gamma-PGA production. Subsequently, C. glutamicum F343 with a strong L-glutamic acid production capability was tested. C. glutamicum F343 carrying capBCA produced gamma-PGA up to 11.4 g/L, showing a higher titer compared with C. glutamicum F343 expressing pgsBCA. By introducing B. subtilis glutamate racemase gene racE under P-tac promoter mutants with different expression strength, the percentage of L-glutamic acid units in gamma-PGA could be adjusted from 97.1% to 36.9%, and stayed constant during the fermentation process, while the gamma-PGA titer reached 21.3 g/L under optimal initial glucose concentrations. The molecular weight (M-w) of gamma-PGA in the engineered strains ranged from 2000 to 4000 kDa. This work provides a foundation for the development of sustainable and cost-effective de novo production of gamma-PGA from glucose with customized ratios of L-glutamic acid in C. glutamicum.
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