Journal
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 106, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.msec.2019.110161
Keywords
Chrysin; Amino-appended beta-cyclodextrin (ACD); Inclusion complex; In vitro antioxidant activity; In vitro cytotoxicity
Categories
Funding
- National Natural Science Foundation of China (NNSFC) [21642001, 81560601]
- Yunnan Applied Basic Research Projects [2018FB018, 2018FA047]
- Natural Science Foundation of Jiangsu Province [BK20160443]
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Solid inclusion complexes between chrysin and four amino-appended beta-cyclodextrins (ACDs) were prepared by suspension method and characterized in solid and solution states by kinds of analytical methods. The scanning electron microscopy (SEM) showed distinct micro-morphologies of them. Thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC) analysis revealed their unique thermal properties, such as decomposition temperatures and endothermic points. Powder X-ray diffractometry (XRD) analysis disclosed their unique crystal patterns. Their nuclear magnetic resonance (NMR) analyses provided the variations of chemical shifts before and after the formation of inclusion complexes. Their binding stability constants (Ks) were 574, 842, 704, and 474 L.mol(-1), respectively, as determined by spectral titration. A 1:1 inclusion mode with self-assembly of their amino side chains inside the ACD cavity was proposed based on Job plot and 2D-ROESY experiments. Water solubility of chrysin was promoted up to 4411.98 mu g.mL(-1) after formation of inclusion complexes with ACDs, better than that of beta-CD and its derivatives, ie., HP- and SBE-beta-CD. In vitro antioxidant activity of chrysin was also improved after inclusion complexation by the DPPH scavenging assay. Furthermore, in vitro cytotoxicity of solid inclusion complexes towards three human cancer cell lines, A549, HT-29 and HCT116 were enhanced significantly.
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