Journal
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 106, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.msec.2019.110268
Keywords
Nanofiber yarn; Core-sheath yarn; Drug delivery; Tenogenic differentiation; Cell migration
Categories
Funding
- Mary & Dick Holland Regenerative Medicine Program start-up grant, Nebraska Research Initiative
- National Institutes of Health [R01 AR073225]
- Chinese science and technology major projects [2017YFB0309805-02, 2018GGX108003]
- Nebraska Research Initiative
- Fred and Pamela Buffett Cancer Center [P30CA036727]
- NIGMS of the NIH [P30GM106397]
Ask authors/readers for more resources
Microfiber yarns (MY) have been widely employed to construct tendon tissue grafts. However, suboptimal ultrastructure and inappropriate environments for cell interactions limit their clinical application. Herein, we designed a modified electrospinning device to coat poly(lactic-co-glycolic acid) PLGA nanofibers onto polylactic acid (PLA) MY to generate PLGA/PLA hybrid yarns (HY), which had a well-aligned nanofibrous structure, resembling the ultrastructure of native tendon tissues and showed enhanced failure load compared to PLA MY. PLGA/PLA HY significantly improved the growth, proliferation, and tendon-specific gene expressions of human adipose derived mesenchymal stem cells (HADMSC) compared to PLA MY. Moreover, thymosin beta-4 (T134) loaded PLGA/PLA HY presented a sustained drug release manner for 28 days and showed an additive effect on promoting HADMSC migration, proliferation, and tenogenic differentiation. Collectively, the combination of T 4 with the nano-topography of PLGA/PLA HY might be an efficient strategy to promote tenogenesis of adult stem cells for tendon tissue engineering.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
