Journal
MARINE ENVIRONMENTAL RESEARCH
Volume 154, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.marenvres.2019.104870
Keywords
Glutathione; Buthionine sulfoximine; Bivalve; Hemocyte immunoparameters; Vibrio; Oxidative stress
Funding
- CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico) [406426/2012-0, 462333/2014-0]
- CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior) [307057/2018-6]
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Glutathione (GSH) is a major cellular antioxidant molecule participating in several biological processes, including immune function. In this study, we investigated the importance of GSH to oysters Crassostrea gigas immune response. Oysters were treated with the GSH-synthesis inhibitor buthionine sulfoximine (BSO), and the function of immune cells and mortality were evaluated after a bacterial challenge with different Vibrio species. BSO caused a moderate decrease (20-40%) in GSH levels in the gills, digestive gland, and hemocytes. As expected, lower GSH decreased survival to peroxide exposure. Hemocyte function was preserved after BSO treatment, however, oysters became more susceptible to challenges with Vibrio anguillarum, V. alginolyticus, or V. harveyi, but not with V. parahaemolyticus and V. vulnificus, indicating a species-specific vulnerability. Our study indicates that in natural habitats or in mariculture farms, disturbances in GSH metabolism may pre-dispose oysters to bacterial infection, decreasing survival.
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