Journal
JOURNAL OF VIROLOGICAL METHODS
Volume 276, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.jviromet.2019.113778
Keywords
TILDA; HIV-1; HIV subtype C; Reservoir quantification
Funding
- European Research Council (ERC) under the European Union's Seventh Framework Programme (FP/2007-2013)/ERC STG [337116]
- Dutch AIDS Fonds [2014021]
- Erasmus MC mRACE grant
- MIUR grant PRIN 2015-2017
- European Research Council (ERC) [337116] Funding Source: European Research Council (ERC)
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A better characterization of the HIV reservoir is pivotal for the development of effective eradication strategies. Accurate quantification of the latent reservoir remains challenging. Starting from a regular blood draw, the Tat/Rev induced limiting dilution assay (TILDA) combines serial dilution of CD4(+) T cells with a PCR-based detection of HIV-1 spliced mRNA produced upon cell stimulation. Here we adapted the original protocol for HIV-1 subtype B to detect tat/rev mRNAs transcribed from reactivated latently infected cells in long term suppressed patients infected with HIV-1 subtype C. Given the heterogeneity of global HIV epidemiology, it is pivotal to develop assays with optimal performances also in patients infected with non-B subtypes. We observed that, in these patients infected with subtype C virus, the HIV reservoir quantified by TILDA correlates with both the time of virological suppression and CD4/CD8 ratio.
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