Journal
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
Volume 31, Issue 4, Pages 873-879Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jasms.9b00122
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Funding
- EPSRC [EP/S002979/1, EP/L023490/1, EP/K039245/1]
- EPSRC via the Centre for Doctoral Training in Physical Sciences for Health (Sci-Phy-4-Health) [EP/L016346/1]
- UCB Pharma
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We have previously demonstrated native liquid extraction surface analysis (LESA) mass spectrometry imaging of small intact proteins in thin tissue sections. We also showed calculation of collision cross sections for specific proteins extracted from discrete locations in tissue by LESA traveling wave ion mobility spectrometry (TWIMS). Here, we demonstrate an integrated native LESA TWIMS mass spectrometry imaging (MSI) workflow, in which ion mobility separation is central to the imaging experiment and which provides spatial, conformational, and mass information on endogenous proteins in a single experiment. The approach was applied to MSI of a thin tissue section of mouse kidney. The results show that the benefits of integration of TWIMS include improved specificity of the ion images and the capacity to calculate collision cross sections for any protein or protein complex detected in any pixel (without a priori knowledge of the presence of the protein).
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