Journal
JOURNAL OF PROTEOME RESEARCH
Volume 19, Issue 3, Pages 1319-1337Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jproteome.9b00851
Keywords
cuticle; cuticular proteins; stylets; acrostyle; virus receptor; proteomics; aphid
Categories
Funding
- Bill and Melinda Gates Foundation (GCEag) [OPP1130147]
- French National Research Agency [ANR15-CE20-0011]
- ERC [APHIDHOST-310190]
- Inra (AIP Bioressources)
- BGI Biotech
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yyyy Aphids are phloem-feeding insects known as major pests in agriculture that are able to transmit hundreds of plant viruses. The majority of these viruses, classified as noncirculative, are retained and transported on the inner surface of the cuticle of the needle-like mouthparts while the aphids move from plant to plant. Identification of receptors of viruses within insect vectors is a key challenge because they are promising targets for alternative control strategies. The acrostyle, an organ discovered earlier within the common food/salivary canal at the tip of aphid maxillary stylets, displays proteins at the cuticle-fluid interface, some of which are receptors of noncirculative viruses. To assess the presence of stylet- and acrostyle-specific proteins and identify putative receptors, we have developed a comprehensive comparative analysis of the proteomes of four cuticular anatomical structures of the pea aphid, stylets, antennae, legs, and wings. In addition, we performed systematic immunolabeling detection of the cuticular proteins identified by mass spectrometry in dissected stylets. We thereby establish the first proteome of stylets of an insect and determine the minimal repertoire of the cuticular proteins composing the acrostyle. Most importantly, we propose a short list of plant virus receptor candidates, among which RR-1 proteins are remarkably predominant. The data are available via ProteomeXchange (PXD016517).
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