Journal
BULLETIN OF MATERIALS SCIENCE
Volume 39, Issue 2, Pages 377-383Publisher
INDIAN ACAD SCIENCES
DOI: 10.1007/s12034-016-1161-7
Keywords
Collagen; chitosan; biocompatibility; adsorption
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Funding
- European Social Fund, Sectorial Operational Programme: Human Resources Development of the Romanian Ministry of Labour, Family and Social Protection through the Financial Agreement POSDRU [132397]
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The aim of this study was to develop a new variant of membranes based on collagen (COL), chitosan (CHI) and alkaline phosphatase (ALP) immobilized and cross-linking with glutaraldehyde (GA) at different concentrations. The biodegradation in the presence of collagenase was investigated. Biocompatibility was evaluated by MTT assay using a mouse fibroblast cell culture type NCTC (clone 929). Non-cross-linked samples were biocompatible and membranes cross-linked with low concentrations of GA (0.04, 0.08%) were also biocompatible. However, high concentrations of GA lead to a decreased biocompatibility. The adsorption behaviour of Ca2+ ions to all membranes were evaluated using the Freundlich isotherms. Haemolytic studies were performed in order to consider their applications in biomineralization process. By the addition of collagen and ALP to chitosan, the haemolytic index decreases, the COL-CHI-ALP membrane being in the non-haemolytic domain, while the COL-CHI-ALP-GA membrane has a haemolytic index greater than 2, and is slightly haemolytic.
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