Journal
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Volume 177, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.jpba.2019.112882
Keywords
Affinity capillary electrophoresis; CE immunoassay; Chiral separations; Biointeraction studies; Enzyme assays
Categories
Funding
- National Institutes of Health (NIH) [RO1 DK069629, RO1 GM044931]
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Affinity capillary electrophoresis (ACE) is a separation technique that combines a biologically-related binding agent with the separating power and efficiency of capillary electrophoresis. This review will examine several classes of binding agents that have been used in ACE and applications that have been described for the resulting methods in clinical or pharmaceutical analysis. Binding agents that will be considered are antibodies, aptamers, lectins, serum proteins, carbohydrates, and enzymes. This review will also describe the various formats in which each type of binding agent has been used in CE, including both homogeneous and heterogeneous methods. Specific areas of applications that will be considered are CE-based immunoassays, glycoprotein/glycan separations, chiral separations, and biointeraction studies. The general principles and formats of ACE for each of these applications will be examined, along with the potential advantages or limitations of these methods. (C) 2019 Elsevier B.V. All rights reserved.
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