Journal
JOURNAL OF ORTHOPAEDIC RESEARCH
Volume 38, Issue 9, Pages 1974-1986Publisher
WILEY
DOI: 10.1002/jor.24644
Keywords
ACLT; cartilage; MRI; osteoarthritis; rabbit model
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Funding
- CIHR Funding Source: Medline
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In this study, the rabbit model with anterior cruciate ligament transection (ACLT) was used to investigate early degenerative changes in cartilage using multiparametric quantitative magnetic resonance imaging (qMRI). ACLT was surgically induced in the knees of skeletally mature New Zealand White rabbits (n = 14). ACL transected and contralateral knee compartments-medial femur, lateral femur, medial tibia, and lateral tibia-were harvested 2 (n = 8) and 8 weeks (n = 6) postsurgery. Twelve age-matched nonoperated rabbits served as control. qMRI was conducted at 9.4 T and included relaxation times T-1, T-2, continuous-wave T-1 rho(CWT1 rho), adiabatic T-1 rho(AdT(1 rho)), adiabatic T-2 rho(AdT(2 rho)), and relaxation along a fictitious field (T-RAFF). For reference, quantitative histology and biomechanical measurements were carried out. Posttraumatic changes were primarily noted in the superficial half of the cartilage. Prolonged T-1, T-2, CWT1 rho, and AdT(1 rho)were observed in the lateral femur 2 and 8 weeks post-ACLT, compared with the corresponding control and contralateral groups (P < .05). Collagen orientation was significantly altered in the lateral femur at 2 weeks post-ACLT compared with the corresponding control group. In the medial femur, all the studied relaxation time parameters, except T-RAFF, were increased 8 weeks post-ACLT, as compared with the corresponding contralateral and control groups (P < .05). Similarly, significant proteoglycan loss was observed in the medial femur at 8 weeks following surgery (P < .05). Multiparametric MRI demonstrated early degenerative changes primarily in the superficial cartilage with T-1, T-2, CWT1 rho, and AdT(1 rho)sensitive to cartilage changes at 2 weeks after surgery.
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