Journal
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
Volume 80, Issue -, Pages -Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jnutbio.2020.108365
Keywords
Amino Acid Profiles; CD36-RXRg; Hepatic Steatosis; Lipid Biosynthesis; Luffa cylindrica; Transcriptome Analysis
Funding
- National Key R&D Program of China [2017YFD0400705]
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Luffa cylindrica is a nutrient-dense vegetable with medical properties and can alleviate metabolic diseases. Numerous evidences demonstrated gut microbiota impacted the progress of nonalcoholic fatty liver disease (NAFLD). This study was to investigate the underlying mechanism of L cylindrica supplementation against NALFD via gut microbiota from hepatic transcriptional and metabolic analysis. In diet-induced obese mice, we observed L cylindrica supplementation (2 g/kg body weight) effectively alleviated high-fat diet-induced obese symptoms such as body weight, fat deposition, and insulin resistance. Notably, L. cylindrica supplementation significantly relieved hepatic steatosis and inflammation infiltration to decrease hepatic toxicity. RNA-sequencing analysis showed that 130 hepatic genes in total significantly altered responding to L cylindrica supplementation. And signaling pathway analysis revealed that L cylindrica supplementation down-regulated the transcriptional expressions of CD36 and Rxrg to inhibit hepatic lipid synthesis. Moreover, L cylindrica supplementation increased the transcriptional expressions of Ass1, Cps1, Cdr. Got1, Tat. and Gls2 to enhance amino acid levels (Gly, Ala, Pro, Val, Ile, Asn, Met, and Phe) and improve hepatic abnormal gluconeogenesis. Furthermore, in antibiotic-treated obese mice, L cylindrica supplementation did not change these gene expressions along with the hepatic levels of lipid and amino adds. Taken together, L cylindrica supplementation could effectively suppress hepatic steatosis in diet-induced obese mice through inhibiting lipid synthesis and enhancing amino acid levels in liver, which depended on gut microbiota. Thus, L cylindrica might be one promising dietary supplementation targeting at gut microbiota to reduce NAFLD risk. (C) 2020 Published by Elsevier Inc.
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