Journal
JOURNAL OF NEUROINFLAMMATION
Volume 17, Issue 1, Pages -Publisher
BMC
DOI: 10.1186/s12974-020-01745-0
Keywords
Subarachnoid hemorrhage; Neuroinflammation; Exosomes; Brain delivery
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Funding
- National Key R&D Program of China [2018YFC1312600, 2018YFC1312601]
- National Natural Science Foundation of China [81701357]
- Anhui Province's Natural Science Foundation [1708085QH181]
- Project of Jiangsu Provincial Medical Innovation Team [CXTDA2017003]
- Jiangsu Provincial Medical Youth Talent [QNRC2016728]
- Suzhou Key Medical Centre [Szzx201501]
- Scientific Department of Jiangsu Province [BE2017656]
- Suzhou Government [LCZX201601]
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Background Inflammation is a potential crucial factor in the pathogenesis of subarachnoid hemorrhage (SAH). Circulating microRNAs (miRNAs) are involved in the regulation of diverse aspects of neuronal dysfunction. The therapeutic potential of miRNAs has been demonstrated in several CNS disorders and is thought to involve modulation of neuroinflammation. Here, we found that peripherally injected modified exosomes (Exos) delivered miRNAs to the brains of mice with SAH and that the potential mechanism was regulated by regulation of neuroinflammation. Methods Next-generation sequencing (NGS) and qRT-PCR were used to define the global miRNA profile of plasma exosomes in aSAH patients and healthy controls. We peripherally injected RVG/Exos/miR-193b-3p to achieve delivery of miR-193b-3p to the brain of mice with SAH. The effects of miR-193b-3p on SAH were assayed using a neurological score, brain water content, blood-brain barrier (BBB) injury, and Fluoro-Jade C (FJC) staining. Western blotting analysis, enzyme-linked immunosorbent assay (ELISA), and qRT-PCR were used to measure various proteins and mRNA levels. Results NGS and qRT-PCR revealed that four circulating exosomal miRNAs were differentially expressed. RVG/Exos exhibited improved targeting to the brains of SAH mice. MiR-193b-3p suppressed the expression and activity of HDAC3, upregulating the acetylation of NF-kappa B p65. Finally, miR-193b-3p treatment mitigated the neurological behavioral impairment, brain edema, BBB injury, and neurodegeneration induced by SAH, and reduced inflammatory cytokine expression in the brains of mice after SAH. Conclusions Exos/miR-193b-3p treatment attenuated the inflammatory response by acetylation of the NF-kappa B p65 via suppressed expression and activity of HDAC3. These effects alleviated neurobehavioral impairments and neuroinflammation following SAH.
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