GDSL esterase/lipases OsGELP34 and OsGELP110/OsGELP115 are essential for rice pollen development

Pollen exine contains complex biopolymers of aliphatic lipids and phenolics. Abnormal development of pollen exine often leads to plant sterility. Molecular mechanisms regulating exine formation have been studied extensively but remain ambiguous. Here we report the analyses of three GDSL esterase/lipase protein genes,OsGELP34,OsGELP110, andOsGELP115, for rice exine formation.OsGELP34was identified by cloning of a male sterile mutant gene.OsGELP34encodes an endoplasmic reticulum protein and was mainly expressed in anthers during pollen exine formation.osgelp34mutant displayed abnormal exine and altered expression of a number of key genes required for pollen development.OsGELP110was previously identified as a gene differentially expressed in meiotic anthers.OsGELP110was most homologous toOsGELP115, and the two genes showed similar gene expression patterns. Both OsGELP110 and OsGELP115 proteins were localized in peroxisomes. Individual knockout ofOsGELP110andOsGELP115did not affect the plant fertility, but double knockout of both genes altered the exine structure and rendered the plant male sterile. OsGELP34 is distant from OsGELP110 and OsGELP115 in sequence, andosgelp34andosgelp110/osgelp115mutants were different in anther morphology despite both were male sterile. These results suggested that OsGELP34 and OsGELP110/OsGELP115 catalyze different compounds for pollen exine development.