Journal
JOURNAL OF EXPERIMENTAL BOTANY
Volume 71, Issue 6, Pages 1757-1762Publisher
OXFORD UNIV PRESS
DOI: 10.1093/jxb/erz543
Keywords
Dynamics; endoplasmic reticulum (ER); ER remodeling; microscopy; movement; quantitative analysis
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Funding
- BBSRC [1810146] Funding Source: UKRI
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The availability of quantification methods for subcellular organelle dynamic analysis has increased rapidly over the last 20 years. The application of these techniques to contiguous subcellular structures that exhibit dynamic remodelling over a range of scales and orientations is challenging, as quantification of 'movement' rarely corresponds to traditional, qualitative classifications of types of organelle movement. The plant endoplasmic reticulum represents a particular challenge for dynamic quantification as it itself is an entirely contiguous organelle that is in a constant state of flux and gross remodelling, controlled by the actinomyosin cytoskeleton.
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